1. Industry Overview
Biological production workshops have two rigid mandatory requirements: biological sterile safe production and industrial explosion-proof safe production. Most traditional conveying equipment cannot satisfy both standards, becoming a core bottleneck restricting production compliance and product quality.
On the one hand, biological materials are extremely sensitive to activity. High-shear conveying equipment directly breaks microbial cell walls, reduces strain activity, causes denaturation of protein molecules, and ultimately leads to lower yield, substandard purity and poor batch stability of biological products. On the other hand, a large number of volatile organic solvents are used in biological extraction and purification processes, which are highly flammable and explosive. Non-explosion-proof motors, exposed wiring and accumulated static electricity from fluid flow are highly likely to trigger flash explosion accidents in closed workshops. In addition, biomedical production strictly implements GMP sterile specifications. Equipment must be free of sanitary dead corners, residual liquid retention and material precipitation, and support high-temperature steam sterilization. Traditional pumps cannot achieve long-term stable sterile operation and fail to pass workshop acceptance and qualification audits.
Therefore, professional explosion-proof sanitary twin screw pumps have become standard conveying equipment for modern biological fermentation, biomedicine and plant biological extraction workshops.
2. Working Principle
As a positive pressure displacement conveying device, the explosion-proof sanitary twin screw pump adopts an external synchronous gear transmission structure. Two conjugate screws rotate synchronously with zero metal contact and zero mechanical friction throughout operation.
During operation, the meshing of screws forms continuous closed volumetric cavities, pushing biological media and organic solvents axially in a stable laminar flow state to realize low-pulse, low-shear and eddy-free stable conveying. The equipment supports bidirectional reversible operation: forward rotation completes process conveying of fermentation liquid and extraction liquid, while reverse rotation realizes recycling of residual solvent and tail liquid in pipelines. The fully closed conveying structure effectively reduces the volatilization of organic solvents, lowers the concentration of combustible gas in the workshop, and improves the explosion-proof safety level of biological workshops from the source.
3. Core Structural Design
3.1 Full-Range Explosion-Proof Safety System
The whole machine is equipped with standardized explosion-proof configurations, adapting to Zone 1 and Zone 2 hazardous working conditions with combustible solvents in biological workshops to fully avoid flammable and explosive safety risks.
Explosion-Proof Motor: Standard Ex d IIB T4 explosion-proof grade, optional upgraded Ex d IIC T6 grade. Fully sealed flameproof structure with IP66 protection rating prevents internal electric spark leakage and adapts to high-humidity workshop environments containing solvent vapor.
Explosion-Proof Wiring System: Equipped with flameproof junction box and explosion-proof cable gland. The fully sealed wiring design effectively blocks solvent vapor from invading electrical components and eliminates potential electrical hazards.
Full-Machine Anti-Static Structure: The pump body, conveying pipeline and anti-static hose form a complete equipotential grounding system, which continuously conducts static electricity generated by high-speed fluid flow to eliminate static ignition risks.
Isolated Gearbox: The synchronous gear cavity is completely isolated from the medium flow channel, preventing volatile solvent vapor from contacting lubricating components and ensuring stable and safe operation of the transmission system.
3.2 GMP Sterile Zero-Dead-Corner Structure
All wetted parts of the equipment are made of passivated 316L/1.4404 stainless steel with mirror electrolytic polishing and a surface roughness of Ra≤0.4μm. The streamlined pump cavity has no right-angle dead corners or liquid storage blind cavities, fundamentally preventing bacterial growth, biofilm accumulation and cross-contamination of materials. The integrally formed screw has no coating or peeling risk, which can stably adapt to long-term high-temperature sterilization in biological workshops and meet GMP sterile production and cleaning verification requirements.
3.3 Biological Exclusive Low-Shear Screw Profile
Adopting a large-pitch screw profile dedicated to biological working conditions, the oversized flow channel allows mycelium clusters, cell clusters and suspended soft particles to pass completely without extrusion and crushing. The ultra-low-shear laminar flow conveying mode maximally retains the activity of microorganisms, proteins and biological enzymes, significantly improving the yield and production stability of biological products.
3.4 Solvent-Resistant Sterile Sealing System
Equipped with double-end mechanical seal with sterile flushing interface as standard, exclusive sealing materials are matched for different biological working conditions to balance sterile performance, solvent resistance and high-temperature resistance:
EPDM: Suitable for fermentation stock solution, cell culture fluid and water-based biological media with no peculiar smell, no precipitation and high sterile safety.
PTFE Encapsulated Seal: Resists corrosion from organic solvents such as ethanol, acetone and isopropanol. It does not swell or produce impurity precipitation after long-term operation, suitable for explosion-proof extraction processes.
HNBR: High temperature resistance and thermal aging resistance, adapting to high-temperature CIP cleaning and high-temperature material conveying working conditions.
4. Exclusive Core Advantages for Biological Production
4.1 Dual Safety Guarantee of Explosion-Proof and Anti-Static Performance
The whole machine is equipped with complete explosion-proof qualifications, complying with GB3836 and IEC international explosion-proof standards. It thoroughly eliminates hidden dangers of electric spark and static ignition, fully meets the safety production specifications of pharmaceutical workshops with combustible solvents, and successfully passes dual acceptance of safety supervision and drug supervision.
4.2 Ultra-Low Shear Protects Biological Activity
Different from the strong stirring and crushing of centrifugal pumps, the twin screw pump adopts pure translational laminar flow conveying with a microbial integrity rate of over 99%. It effectively avoids protein denaturation, cell rupture and loss of active ingredients, which is crucial for the quality guarantee of high-value biological products such as vaccines, monoclonal antibodies and high-end biochemical raw materials.
4.3 Full Compatibility with CIP/SIP Sterilization
The entire flow channel supports online closed-loop CIP cleaning and 135℃ instantaneous high-temperature SIP steam sterilization. Full-range sterilization can be completed without disassembly, with no cleaning dead corners, fully complying with GMP cleaning verification and sterile production standards.
4.4 Pulse-Free Stable Conveying Improves Finished Product Qualification Rate
Constant flow and pressure without fluctuation stabilize the liquid level and pressure in precision processes such as biological chromatography, purification and filling, greatly improving batch consistency and finished product qualification rate of products.
4.5 Wide Viscosity Adaptability, Multi-Function to Reduce Costs and Improve Efficiency
It can stably convey all types of biological media including low-viscosity buffer solution, medium-viscosity fermentation liquid and high-viscosity mycelium paste. A single piece of equipment covers the whole processes of fermentation, extraction, purification and cleaning, significantly reducing the number of workshop equipment purchases, spare parts inventory costs and operation and maintenance difficulties.
5. Typical Application Scenarios in Biological Industry
Biological Fermentation Industry: Yeast fermentation, amino acid fermentation, microbial stock solution transfer and mycelium concentration conveying.
Biomedical GMP Workshops: Conveying and sterile filling of vaccine culture fluid, pharmaceutical protein liquid, antibody stock solution and sterile buffer solution.
Plant Biological Extraction Workshops: Ethanol extraction, acetone elution, organic solvent recovery and concentration conveying of natural active ingredients.
Biochemical Raw Material Production: Conveying high-purity biological raw materials such as polypeptides, biological enzymes and polysaccharides.
Laboratory and Pilot Production: Explosion-proof and sterile conveying for small fermentation tanks and experimental purification production lines.
6. Standard Technical Parameters
Flow Range: 0.3–100 m³/h
Working Pressure: 0.2–1.8 MPa
Medium Temperature: -10℃～95℃; Instantaneous 135℃ (SIP sterilization)
Explosion-Proof Grade: Standard Ex d IIB T4, optional Ex d IIC T6
Surface Finish: Ra≤0.4μm (GMP sterile grade)
Microbial Integrity Rate: ≥99%
Protection Grade: IP66
Applicable Viscosity: 1–1500 cPs